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Quantitative real-time PCR (RT-qPCR) summarizing the gene expression of all isolates in early-phase and late-phase infection. Gene expression levels were measured at 0, 12, 24, and 48 h post-infection. ■ represents the early-phase infection, and ○ represents the late-phase infection. ( A ) IL1B expression significantly declines from early to late phase infction. ( B ) IL6 and ( C ) TNF showes gradually increasing expression of pro-inflammatory cytokines compared from early to late phase infection ( D ) ULBP2 expression demonstrates decrease expression over time. ( E ) IFNB1 markedly induced a gradually increased expression compared from early to late phase infection. ( F ) <t>IFNAR2</t> remains slight downward expression trend. ( G ) DDX58 increasing more prominently in the early phase, particularly at later time points. ( H ) TMPRSS2 and ( I ) ACE2, both as the cell entry genes demonstrate expression across both phases, especially ACE2 shows significantly downregulated over time compared from early to late phase infection. Statistical significance is tested using a t -test. The error bars indicate the mean ± SD. (*) indicates a significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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Quantitative real-time PCR (RT-qPCR) summarizing the gene expression of all isolates in early-phase and late-phase infection. Gene expression levels were measured at 0, 12, 24, and 48 h post-infection. ■ represents the early-phase infection, and ○ represents the late-phase infection. ( A ) IL1B expression significantly declines from early to late phase infction. ( B ) IL6 and ( C ) TNF showes gradually increasing expression of pro-inflammatory cytokines compared from early to late phase infection ( D ) ULBP2 expression demonstrates decrease expression over time. ( E ) IFNB1 markedly induced a gradually increased expression compared from early to late phase infection. ( F ) <t>IFNAR2</t> remains slight downward expression trend. ( G ) DDX58 increasing more prominently in the early phase, particularly at later time points. ( H ) TMPRSS2 and ( I ) ACE2, both as the cell entry genes demonstrate expression across both phases, especially ACE2 shows significantly downregulated over time compared from early to late phase infection. Statistical significance is tested using a t -test. The error bars indicate the mean ± SD. (*) indicates a significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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Quantitative real-time PCR (RT-qPCR) summarizing the gene expression of all isolates in early-phase and late-phase infection. Gene expression levels were measured at 0, 12, 24, and 48 h post-infection. ■ represents the early-phase infection, and ○ represents the late-phase infection. ( A ) IL1B expression significantly declines from early to late phase infction. ( B ) IL6 and ( C ) TNF showes gradually increasing expression of pro-inflammatory cytokines compared from early to late phase infection ( D ) ULBP2 expression demonstrates decrease expression over time. ( E ) IFNB1 markedly induced a gradually increased expression compared from early to late phase infection. ( F ) IFNAR2 remains slight downward expression trend. ( G ) DDX58 increasing more prominently in the early phase, particularly at later time points. ( H ) TMPRSS2 and ( I ) ACE2, both as the cell entry genes demonstrate expression across both phases, especially ACE2 shows significantly downregulated over time compared from early to late phase infection. Statistical significance is tested using a t -test. The error bars indicate the mean ± SD. (*) indicates a significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: Host–Virus Interface in Persistent SARS-CoV-2 Infections: Viral Characteristic Evolution and Gene Expression Profiling Analysis

doi: 10.3390/ijms26136221

Figure Lengend Snippet: Quantitative real-time PCR (RT-qPCR) summarizing the gene expression of all isolates in early-phase and late-phase infection. Gene expression levels were measured at 0, 12, 24, and 48 h post-infection. ■ represents the early-phase infection, and ○ represents the late-phase infection. ( A ) IL1B expression significantly declines from early to late phase infction. ( B ) IL6 and ( C ) TNF showes gradually increasing expression of pro-inflammatory cytokines compared from early to late phase infection ( D ) ULBP2 expression demonstrates decrease expression over time. ( E ) IFNB1 markedly induced a gradually increased expression compared from early to late phase infection. ( F ) IFNAR2 remains slight downward expression trend. ( G ) DDX58 increasing more prominently in the early phase, particularly at later time points. ( H ) TMPRSS2 and ( I ) ACE2, both as the cell entry genes demonstrate expression across both phases, especially ACE2 shows significantly downregulated over time compared from early to late phase infection. Statistical significance is tested using a t -test. The error bars indicate the mean ± SD. (*) indicates a significant difference, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: IFNAR2 , Hs01022059_m1 , 88.

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Infection, Expressing